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KMID : 0355519810050010041
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Korean Journal of Oral Biology
1981 Volume.5 No. 1 p.41 ~ p.48
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EFFECTS OF STEROID HORMONES AND FLUORIDE ON THE COLLAGEN BIOSYNTHESIS IN EXPERIMENTAL RAT BONE TISSUES
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Abstract
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Effects of steroid hormones and fluoride on the collagen biosynthesis in femur and upper alveolar bone were studied with overiectomized Sprague-Dawley rats. Effects of administration cf hormones such as estrogen, testosterone, prednisolone, and fluoride to the overiectomized animals were studied, comparing with the control. Each group was injected with ^(3)H-proline and sacrificed, followed by removals of femur and upper alveolar bone. Separations and quantitative analyses of proline and hydroxyproline were performed by means of thin layer chromatography and radioactivities of the separated amino acids were assayed by liquid scintillation counter.
The results were as follows.
1. Normally the incorporation of ^(3)H-proline into hydroxyproline was greater in distal femur than in shaft femur and upper alveolar bone.
2. In the tissues from ovariectomized rats, the incorporation rate of ^(3)H-proline into hydroxyproline was decreased than that of the normal.
3. Decrease in ^(3)H-proline incorporation into collagen in ovariectomized rats was antagonized by estrogen and testosterone but augmented by prednisolone in the tissues tested. The effect of ovariectomy on collagen synthesis was augmented by fluoride in all the tissues except distal femur.
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